Descriptions

In HPLC analysis, the particle size of the chromatographic column packing is small and it is easy to be blocked by impurity particles. Therefore, samples and solvents need to be filtered in advance to remove particulate contaminants and protect the instrument.

Ion chromatography, which is commonly used in environmental analysis, also requires that no inorganic pollutants be introduced in sample pretreatment.

Syringe filters can be used in HPLC analysis and IC analysis to filter sample solutions, which is an important step in the sample pretreatment process.

Membrane Characteristics

The following chart will help you quickly choose the proper filter membrane type.

Syringe Filter Diameter:

13 mm Syringe Filters

For sample volumes <10 mL range;

Holdup (dead) volume is <30 ul.

Maximum operation pressure <100psi

Filter area: 0.85cm2

25 mm Syringe Filters

For sample volumes <100 mL range;

Holdup (dead) volume is <100 ul.

Maximum operation pressure <89 psi

Filter area: 4.3cm2

Syringe Filter Membrane Pore Size:

0.22um: Sterilization grade filter membrane, sometimes written as 0.2um, can remove very fine particles in samples and mobile phases; it can meet the requirements of 99.99% sterilization specified by GMP or Pharmacopoeia;

0.45μm: usually used for pretreatment to reduce the microbial load and filter out most bacteria and microorganisms; conventional sample and mobile phase filtration can meet general chromatographic requirements;

1-5μm: To filter larger particles of impurities, or for the pretreatment of difficult-to-handle turbid solutions, it can be filtered with a 1-5μm membrane first, and then filtered with the corresponding membrane.

Applications:

• HPLC aqueous sample preparation

• Biological sample preparation

• Buffer solutions

• Salt solutions

• Tissue culture media

• Irrigation solutions

• Sterile isolation

• Medical use, sterile filtering protein solution, tissue culture media, additives.

Related Service

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